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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Bloom Syndrome (Phospho-Thr99) Antibody   

Bloom Syndrome (Phospho-Thr99) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Bloom Syndrome (Phospho-Thr99) Antibody AP52409
    Western blot analysis of extracts from HepG2 cells, using Bloom Syndrome Protein (Phospho-Thr99) antibody.
  • IHC - Bloom Syndrome (Phospho-Thr99) Antibody AP52409
    Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P54132
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 159000 Da
Additional Information
Gene ID 641
Other Names Bloom syndrome protein, DNA helicase, RecQ-like type 2, RecQ2, RecQ protein-like 3, BLM, RECQ2, RECQL3
Dilution WB~~1:1000
IHC~~1:50~100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name BLM
Synonyms RECQ2, RECQL3
Function Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Cellular Location Nucleus. Note=Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner
Research Areas
Citations (0)

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Background

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).

References

Ellis N.A.,et al.Cell 83:655-666(1995).
Karow J.K.,et al.J. Biol. Chem. 272:30611-30614(1997).
Kaneko H.,et al.Biochem. Biophys. Res. Commun. 240:348-353(1997).
Wang Y.,et al.Genes Dev. 14:927-939(2000).
Pichierri P.,et al.EMBO J. 23:3154-3163(2004).

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$ 295.00
Cat# AP52409
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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