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Cortactin (Phospho-Tyr466) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Cortactin (Phospho-Tyr466) Antibody AP52453
    Western blot analysis of extracts from 293 cells using Cortactin (phospho-Tyr466) antibody.
  • WB - Cortactin (Phospho-Tyr466) Antibody AP52453
    Western blot analysis of extracts from COS7 cells (Lane 2) and JK cells (Lane 3), using Cortactin (Phospho-Tyr466) Antibody. The lane on the left is treated with synthesized peptide.
  • IHC - Cortactin (Phospho-Tyr466) Antibody AP52453
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin (phospho-Tyr466) antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q60598
Other Accession Q14247
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 61250 Da
Additional Information
Gene ID 13043
Other Names Src substrate cortactin, Cttn, Ems1
Dilution WB~~1:1000
IHC~~1:50~100
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name Cttn
Synonyms Ems1
Function Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902). Plays a role in the invasiveness of cancer cells, and the formation of metastases (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1- phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cytoplasm, cell cortex Cell projection {ECO:0000250|UniProtKB:Q66HL2}. Cell projection, podosome {ECO:0000250|UniProtKB:Q01406}. Cell projection, dendritic spine. Cell projection, dendrite. Cell membrane {ECO:0000250|UniProtKB:Q66HL2}; Peripheral membrane protein; Cytoplasmic side. Cell junction {ECO:0000250|UniProtKB:Q66HL2} Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:Q66HL2}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:Q66HL2} Note=Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers. In the presence of CTTNBP2, localizes at the cell cortex. In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft. Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity) {ECO:0000250|UniProtKB:Q14247, ECO:0000250|UniProtKB:Q66HL2}
Tissue Location Expressed in most tissues, except in B- lymphocytes or plasma cells
Research Areas
Citations (0)

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Background

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902). Plays a role in the invasiveness of cancer cells, and the formation of metastases (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1- phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity).

References

Miglarese M.R.,et al.Oncogene 9:1989-1997(1994).
Zhan X.,et al.J. Biol. Chem. 268:24427-24431(1993).
Carninci P.,et al.Science 309:1559-1563(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Cavallaro U.,et al.Nat. Cell Biol. 3:650-657(2001).

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$ 295.00
Cat# AP52453
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(40 western blots)
Availability: 5-7 days
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