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RSAD1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RSAD1 Antibody (C-term) AP5262B
    Western blot analysis of RSAD1 Antibody (C-term) (Cat. #AP5262b) in HepG2,NCI-H460 cell line lysates (35ug/lane).RSAD1 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - RSAD1 Antibody (C-term) AP5262B
    RSAD1 Antibody (C-term) (Cat. #AP5262b) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSAD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - RSAD1 Antibody (C-term) AP5262B
    RSAD1 Antibody (C-term) (Cat. #AP5262b) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q9HA92
Other Accession Q5SUV1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48714 Da
Antigen Region 299-328 aa
Additional Information
Gene ID 55316
Other Names Radical S-adenosyl methionine domain-containing protein 1, mitochondrial, 1399-, Oxygen-independent coproporphyrinogen-III oxidase-like protein RSAD1, RSAD1
Target/Specificity This RSAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-328 amino acids from the C-terminal region of human RSAD1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRSAD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RSAD1
Function May be a heme chaperone, appears to bind heme. Homologous bacterial proteins do not have oxygen-independent coproporphyrinogen- III oxidase activity (Probable). Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine (By similarity).
Cellular Location Mitochondrion.
Research Areas
Citations (0)
citation

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Background

RSAD1 may be involved in porphyrin cofactor biosynthesis.

References

Suzuki, Y., et al. Gene 200 (1-2), 149-156 (1997)

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$ 365.00
$ 140.00
Cat# AP5262B
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