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Sox2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - Sox2 Antibody AP52658
    Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.
  • ICC - Sox2 Antibody AP52658
    Immunocytochemistry of COS7 cells using anti-Sox2 mouse mAb diluted 1:150.
  • FC - Sox2 Antibody AP52658
    Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, FC
Primary Accession P48431
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 35 KDa
Additional Information
Gene ID 6657
Other Names ANOP3;cb236;Delta EF2a;lcc;MCOPS3;MGC148683;MGC2413;RGD1565646;Sex determining region Y box 2;SOX 2;Sox2;SOX2_HUMAN;SRY (sex determining region Y) box 2;SRY box containing gene 2;SRY related HMG box 2;SRY related HMG box gene 2;SRY-box 2;Transcription factor SOX 2;Transcription factor SOX-2;ysb.
Dilution WB~~1:1000
ICC~~1:150
FC~~1:100
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SOX2
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

References

Stevanovic M.,et al.Mamm. Genome 5:640-642(1994).
Sadler L.A.,et al.Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases.
Fantes J.,et al.Nat. Genet. 33:461-463(2003).
Takahashi K.,et al.Cell 131:861-872(2007).
Rigbolt K.T.,et al.Sci. Signal. 4:RS3-RS3(2011).

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$ 295.00
Cat# AP52658
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
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