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>   home   >   Products   >   Primary Antibodies   >   BiP/GRP78 (C-terminus) Antibody   

BiP/GRP78 (C-terminus) Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - BiP/GRP78 (C-terminus) Antibody AP52682
    Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.
  • ICC - BiP/GRP78 (C-terminus) Antibody AP52682
    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).
  • IHC - BiP/GRP78 (C-terminus) Antibody AP52682
    Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P11021
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 78 KDa
Additional Information
Gene ID 3309
Other Names 78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP; D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum lumenal Ca2+ binding protein grp78;FLJ26106;Glucose Regulated Protein 78kDa;GRP 78;GRP-78;GRP78;GRP78_HUMAN;Heat shock 70 kDa protein 5;Heat Shock 70kDa Protein 5;Hsce70;HSPA 5;HSPA5;Immunoglobulin Heavy Chain Binding Protein; Immunoglobulin heavy chain-binding protein;mBiP;MIF2;Sez7.
Dilution WB~~1:1000
ICC~~1:50
IHC~~1:100
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HSPA5
Synonyms GRP78
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Cellular Location Endoplasmic reticulum lumen. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Citations (0)

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Background

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

References

Ting J.,et al.DNA 7:275-286(1988).
Chao C.C.K.,et al.Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
Hansen J.J.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Bermudez-Fajardo A.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).

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$ 295.00
Cat# AP52682
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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