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DNA-PKcs Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - DNA-PKcs Antibody AP52744
    Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.
  • ICC - DNA-PKcs Antibody AP52744
    Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:100).
  • IP - DNA-PKcs Antibody AP52744
    Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.
  • IHC - DNA-PKcs Antibody AP52744
    Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, IP, IHC
Primary Accession P78527
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 450 KDa
Additional Information
Gene ID 5591
Other Names DNA dependent protein kinase catalytic subuni;DNA PKcs;DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNAPK;DNPK1;hyper radiosensitivity of murine scid mutation, complementing 1;HYRC;HYRC1;p350;p460;PRKDC;PRKDC_HUMAN;protein kinase, DNA activated, catalytic polypeptide;XRCC7.
Dilution WB~~1:1000
ICC~~1:100
IP~~1:500
IHC~~1:100
Format Purified mouse monoclonal antibody in PBS(pH 7.4)containing with 0.02% sodium azide,0.1%BSA & 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PRKDC
Synonyms HYRC, HYRC1
Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.
Cellular Location Nucleus Nucleus, nucleolus
Research Areas
Citations (0)

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Background

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.

References

Hartley K.O.,et al.Cell 82:849-856(1995).
Gell D.,et al.Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
Anderson C.W.,et al.Radiat. Res. 156:2-9(2001).
Ladenburger E.M.,et al.Cytogenet. Cell Genet. 77:268-270(1997).
Sipley J.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:7515-7519(1995).

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$ 295.00
Cat# AP52744
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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