|Application ||WB, ICC|
|Calculated MW||25 KDa|
|Other Names||UCHL1 / PGP9.5;UCHL1;B220;CD 45;CD45;cd45 antigen;ec22.214.171.124;GP 180;GP180;Human homolog of severe combined immunodeficiency due to PTPRC deficiency;L CA;L-CA;lca;Leukocyte common antigen;LY 5;LY5;Protein tyrosine phosphatase receptor type C;Protein tyrosine phosphatase receptor type c polypeptide;PTPRC;PTPRC_HUMAN;Receptor-type tyrosine-protein phosphatase C;SCID due to PTPRC deficiency;t200;T200 glycoprotein;T200 leukocyte common antigen.|
|Format||Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50% glycerol|
|Storage||Store at -20 °C.Stable for 12 months from date of receipt|
|Function||Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.|
|Cellular Location||Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain|
|Tissue Location||Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients|
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Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Day I.N.M.,et al.Biochem. J. 268:521-524(1990).
Choi J.,et al.J. Biol. Chem. 279:13256-13264(2004).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
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