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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AMPK beta 1 Antibody   

AMPK beta 1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - AMPK beta 1 Antibody AP52790
    Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.
  • ICC - AMPK beta 1 Antibody AP52790
    Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).
  • IP - AMPK beta 1 Antibody AP52790
    Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.
  • IHC - AMPK beta 1 Antibody AP52790
    Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, IP, IHC
Primary Accession Q9Y478
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 38 KDa
Additional Information
Gene ID 5564
Other Names 1300015D22Rik;5 AMP activated protein kinase subunit beta 1;5''-AMP-activated protein kinase subunit beta-1;AAKB1_HUMAN;AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1; AMP-activated, noncatalytic, beta-1;AMPK;AMPK beta 1 chain;AMPK subunit beta-1;AMPK-BETA-1;AMPKb;AU021155;E430008F22;HAMPKb;MGC17785;PRKAB1.
Dilution WB~~1:1000
ICC~~1:100
IP~~1:500
IHC~~1:100
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PRKAB1
Synonyms AMPK
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Research Areas
Citations (0)

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Background

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

References

Carling D.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Stapleton D.,et al.FEBS Lett. 409:452-456(1997).
Yamagata K.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
Wang X.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
Scherer S.E.,et al.Nature 440:346-351(2006).

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$ 295.00
Cat# AP52790
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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