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FEN-1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - FEN-1 Antibody AP52835
    Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
    detail
  • ICC - FEN-1 Antibody AP52835
    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession P39748
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 45 KDa
Additional Information
Gene ID 2237
Other Names DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2.
Dilution WB~~1:1000
ICC~~1:400
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.09% (W/V) sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name FEN1 {ECO:0000255|HAMAP-Rule:MF_03140}
Synonyms RAD2
Function Structure-specific nuclease with 5'-flap endonuclease and 5'- 3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site- terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Cellular Location [Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage
Research Areas
Citations (0)
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Background

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

References

Murray J.M.,et al.Mol. Cell. Biol. 14:4878-4888(1994).
Hiraoka L.R.,et al.Genomics 25:220-225(1995).
Taylor T.D.,et al.Nature 440:497-500(2006).
Robins P.,et al.J. Biol. Chem. 269:28535-28538(1994).
Shen B.,et al.J. Biol. Chem. 271:9173-9176(1996).

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$ 360.00
Cat# AP52835
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