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ZAP128 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP5301c 0.1 mg 400 ul In Stock $ 255.00 Add to cart
AP5301c-ev20 Ev20 size 100 ul In Stock $ 95.00 Add to cart
  • Specification
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ZAP128 Antibody (Center) - Product info

ApplicationWB, IHC, FC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP49753
Other AccessionQ86TX2, NP_006812.3
ReactivityHuman
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW53218 Da

ZAP128 Antibody (Center) - Additional info

Gene ID 10965
Other Names
ACOT2; PTE2; PTE2A; Acyl-coenzyme A thioesterase 2, mitochondrial; Acyl-coenzyme A thioester hydrolase 2a; CTE-Ia; Long-chain acyl-CoA thioesterase 2; ZAP128
Target/Specificity
This ZAP128 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Central region of human ZAP128.
Dilution
WB~~1:100~500
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
ZAP128 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

ZAP128 Antibody (Center) - Protein Information

Name ACOT2
Synonyms PTE2, PTE2A
Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH Displays high levels of activity on medium- and long chain acyl CoAs
Cellular Location
Mitochondrion.
Tissue Location
Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas

ZAP128 Antibody (Center) - Related products

AP5301c: ZAP128 Antibody (Center)

AP5652c: ACOT2 Antibody (Center)

BP5301c: ZAP128 Antibody (Center) Blocking peptide

BP5652c: ACOT2 Antibody (Center) Blocking peptide

ZAP128 Antibody (Center) - Application data

  • Western blot analysis of ZAP128 Antibody (Center) (Cat. #AP5301c) in MCF-7 cell line lysates (35ug/lane).ZAP128 (arrow) was detected using the purified Pab.

  • ZAP128 Antibody (Center) (Cat. #AP5301c) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZAP128 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

  • ZAP128 Antibody (Center) (Cat. #AP5301c) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ZAP128 Antibody (Center) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Acyl-CoA thioesterases, such as ACOT2, are a group of enzymes that hydrolyze CoA esters, such as acyl-CoAs, bile CoAs, and CoA esters of prostaglandins, to the corresponding free acid and CoA.

REFERENCES

Mandel, C.R., et al. Biochem. Biophys. Res. Commun. 385(4):630-633(2009) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) Hunt, M.C., et al. FASEB J. 20(11):1855-1864(2006)