|Application ||WB, IHC-P, FC, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||49910 Da|
|Antigen Region||111-139 aa|
|Other Names||E3 ubiquitin-protein ligase MYLIP, 632-, Inducible degrader of the LDL-receptor, Idol, Myosin regulatory light chain interacting protein, MIR, MYLIP, BZF1, IDOL|
|Target/Specificity||This MYLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the Central region of human MYLIP.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MYLIP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein|
|Tissue Location||Ubiquitously expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.
Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009)
Lindholm, D., et al. Cell. Mol. Life Sci. 66(21):3399-3402(2009)
Zelcer, N., et al. Science 325(5936):100-104(2009)
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