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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MYLIP Antibody (Center)   

MYLIP Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MYLIP Antibody (Center) AP5315c
    Anti-MYLIP Antibody (Center) at 1:2000 dilution + Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MYLIP Antibody (Center) AP5315c
    All lanes : Anti-MYLIP Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MYLIP Antibody (Center) AP5315c
    Western blot analysis of lysate from mouse kidney tissue lysate, using MYLIP Antibody (Center)(Cat. #AP5315c). AP5315c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - MYLIP Antibody (Center) AP5315c
    MYLIP Antibody (Center T126) (Cat. #AP5315c) western blot analysis in mouse heart tissue lysates (35ug/lane). This demonstrates the MYLIP antibody detected the MYLIP protein (arrow).
  • IHC-P - MYLIP Antibody (Center) AP5315c
    MYLIP antibody (Center) (Cat. #AP5315c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the MYLIP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - MYLIP Antibody (Center) AP5315c
    MYLIP Antibody (Center) (Cat. #AP5315c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WY64
Other Accession NP_037394
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49910 Da
Antigen Region 111-139 aa
Additional Information
Gene ID 29116
Other Names E3 ubiquitin-protein ligase MYLIP, 632-, Inducible degrader of the LDL-receptor, Idol, Myosin regulatory light chain interacting protein, MIR, MYLIP, BZF1, IDOL
Target/Specificity This MYLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-139 amino acids from the Central region of human MYLIP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYLIP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BZF1, IDOL
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth.


Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009)
Lindholm, D., et al. Cell. Mol. Life Sci. 66(21):3399-3402(2009)
Zelcer, N., et al. Science 325(5936):100-104(2009)

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Cat# AP5315c
(40 western blots)
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