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Bmi1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - Bmi1 Antibody AP53265
    Western blot detection of Bmi1 in Hela,A549 and 293T cell lysates using Bmi1 mouse mAb (1:1000 diluted).Predicted band size:43KDa.Observed band size:43KDa.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P35226
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 43 KDa
Additional Information
Gene ID 100532731;648
Other Names B lymphoma Mo MLV insertion region (mouse);B lymphoma Mo MLV insertion region 1 homolog;Bmi 1;BMI1;BMI1 polycomb ring finger oncogene;BMI1_HUMAN;Flvi 2/bmi 1; FLVI2/BMI1;MGC12685;Murine leukemia viral (bmi 1) oncogene homolog;Oncogene BMI 1;PCGF 4;PCGF4;Polycomb complex protein BMI 1;Polycomb complex protein BMI-1;Polycomb group protein Bmi1;Polycomb group ring finger 4;Polycomb group RING finger protein 4;RING finger protein 51;RNF 51;RNF51.
Dilution WB~~1:1000
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name BMI1
Synonyms PCGF4, RNF51
Function Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Cellular Location Nucleus. Cytoplasm.
Research Areas
Citations (0)

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Background

Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

References

Alkema M.J.,et al.Hum. Mol. Genet. 2:1597-1603(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Levy L.S.,et al.Oncogene 8:1833-1838(1993).

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$ 295.00
Cat# AP53265
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
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