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beta Actin Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - beta Actin Antibody AP53270
    Western blot detection of beta actin in Hela,3T3,COS7,CHO-k1 and Goat muscle cell lysates using beta actin mouse mAb (1:1500 diluted).Predicted band size:45KDa.Observed band size:45KDa.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P60709
Reactivity Human, Mouse, Neisseria Gonorrhoeae
Host Mouse
Clonality Monoclonal
Isotype IgG3
Calculated MW 45 KDa
Additional Information
Gene ID 60
Other Names A X actin like protein;ACTB;ACTB_HUMAN;Actin;Actin cytoplasmic 1;Actx;b actin;b-actin;Beta cytoskeletal actin;Beta-actin;cytoplasmic 1;Melanoma X actin;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1.
Dilution WB~~1:1500
Format Purified mouse monoclonal in PB(pH 7.4) containing with 0.09% (W/V) sodium azide,50% glycerol and 1mg/ml BSA.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACTB
Function Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas
Citations (0)
citation

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Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

References

Ponte P.,et al.Nucleic Acids Res. 12:1687-1696(1984).
Nakajima-Iijima S.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985).
Ohmori H.,et al.J. Cell Biol. 116:933-941(1992).
Hillier L.W.,et al.Nature 424:157-164(2003).
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).

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$ 360.00
Cat# AP53270
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