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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PP15 Antibody   

PP15 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PP15 Antibody AP53312
    Anti-PP15 Antibody at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P61970
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 14 KDa
Additional Information
Gene ID 10204
Other Names Nuclear transport factor 2, NTF-2, Placental protein 15, PP15, NUTF2, NTF2
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NUTF2 (HGNC:13722)
Function Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.
Cellular Location Cytoplasm, cytosol. Nucleus outer membrane {ECO:0000250|UniProtKB:P61972}. Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P61972}. Nucleus inner membrane {ECO:0000250|UniProtKB:P61972}. Nucleus, nucleoplasm. Note=At steady state it is essentially nucleoplasmic, enriched in nucleoplasmic foci
Research Areas
Citations (0)

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Background

Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.

References

Grundmann U.,et al.Nucleic Acids Res. 16:4721-4721(1988).
Paschal B.M.,et al.J. Cell Biol. 129:925-937(1995).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
Cat# AP53312
Size:
Quantity:
(40 western blots)
Availability: 5-7 days
Bulk Size
Seasonal Special on Bulk Order
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