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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IDUA Antibody (Center)   

IDUA Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - IDUA Antibody (Center) AP5343c
    Overlay histogram showing Hela cells stained with AP5343c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5343c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - IDUA Antibody (Center) AP5343c
    Anti-IDUA Antibody (Center) at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • FC - IDUA Antibody (Center) AP5343c
    Overlay histogram showing HepG2 cells stained with AP5343c (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5343c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - IDUA Antibody (Center) AP5343c
    All lanes : Anti-IDUA Antibody (Center) at 1:2000 dilution Lane 1: human liver lysates Lane 2: human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IDUA Antibody (Center) AP5343c
    Anti-IDUA Antibody (Center)at 1:2000 dilution + human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IDUA Antibody (Center) AP5343c
    Western blot analysis of lysates from A549 cell line, rat lung tissue lysate (from left to right), using IDUA Antibody (Center)(Cat. #AP5343c). AP5343c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • WB - IDUA Antibody (Center) AP5343c
    IDUA Antibody (Center) (Cat. #AP5343c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the IDUA antibody detected the IDUA protein (arrow).
  • IHC-P - IDUA Antibody (Center) AP5343c
    IDUA Antibody (Center) (Cat. #AP5343c) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IDUA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P35475
Other Accession NP_000194.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 72670 Da
Antigen Region 236-264 aa
Additional Information
Gene ID 3425
Other Names Alpha-L-iduronidase, IDUA
Target/Specificity This IDUA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human IDUA.
Dilution FC~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIDUA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IDUA
Cellular Location Lysosome.
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Background

IDUA encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans.

References

Amr, K., et al. Genet Test Mol Biomarkers 13(6):761-764(2009)
Vazna, A., et al. Am. J. Med. Genet. A 149A (5), 965-974 (2009)
Sugawara, K., et al. J. Hum. Genet. 53(5):467-474(2008)

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Discontinued
Cat# AP5343c
Size:
(40 western blots)

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