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C14orf179 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - C14orf179 Antibody (Center) AP5370c
    C14orf179 Antibody (Center)(Cat. #AP5370c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the C14orf179 antibody detected the C14orf179 protein (arrow).
    detail
  • IHC-P - C14orf179 Antibody (Center) AP5370c
    C14orf179 Antibody (Center) (Cat. #AP5370c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C14orf179 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - C14orf179 Antibody (Center) AP5370c
    C14orf179 Antibody (Center) (Cat. #AP5370c) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q96FT9
Other Accession Q9DA69, Q2TBN9, NP_001096034.1, NP_443105.2
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23529 Da
Antigen Region 145-171 aa
Additional Information
Gene ID 112752
Other Names Intraflagellar transport protein 43 homolog, IFT43, C14orf179
Target/Specificity This C14orf179 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-171 amino acids from the Central region of human C14orf179.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC14orf179 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IFT43 (HGNC:29669)
Synonyms C14orf179
Function As a component of IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein- coupled receptors (GPCRs), it is involved in ciliogenesis (PubMed:28400947, PubMed:28973684). Involved in retrograde ciliary transport along microtubules from the ciliary tip to the base (PubMed:21378380).
Cellular Location Cytoplasm, cytoskeleton. Cell projection, cilium Note=Associated with microtubules (PubMed:22361696). Localized at the distal tip of the cilium (PubMed:28973684)
Tissue Location Expressed in the retina, predominantly in the photoreceptor outer segment.
Research Areas
Citations (0)
citation

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References

Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Heilig, R., et al. Nature 421(6923):601-607(2003)

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$ 365.00
$ 140.00
Cat# AP5370c
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