|Application ||WB, IHC-P, FC, E|
|Other Accession||Q16666, NP_945146.1, NP_945148.1|
|Calculated MW||55065 Da|
|Antigen Region||26-55 aa|
|Other Names||Pyrin and HIN domain-containing protein 1, Interferon-inducible protein X, PYHIN1, IFIX|
|Target/Specificity||This PYHIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PYHIN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PYHIN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.|
|Cellular Location||Isoform 1: Nucleus, nucleoplasm. Isoform 5: Nucleus. Nucleus speckle.|
|Tissue Location||Expressed in spleen, lymph node and peripheral blood leukocytes, and at lower levels in thymus, bone marrow and fetal liver. Down-regulated in breast tumors|
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Provided below are standard protocols that you may find useful for product applications.
PYHIN1 belongs to the HIN200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-terminal ends. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis (Ding et al., 2006 [PubMed 16479015]).
Yamaguchi, H., et al. Mol. Carcinog. 47(10):739-743(2008)
Ding, Y., et al. Mol. Cell. Biol. 26(5):1979-1996(2006)
Ding, Y., et al. Oncogene 23(26):4556-4566(2004)
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