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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   PYHIN1 Antibody (N-term)   

PYHIN1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PYHIN1 Antibody (N-term) AP5376a
    PYHIN1 Antibody (N-term)(Cat. #AP5376a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PYHIN1 antibody detected the PYHIN1 protein (arrow).
  • IHC-P - PYHIN1 Antibody (N-term) AP5376a
    PYHIN1 Antibody (N-term) (Cat. #AP5376a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYHIN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PYHIN1 Antibody (N-term) AP5376a
    PYHIN1 Antibody (N-term) (Cat. #AP5376a) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6K0P9
Other Accession Q16666, NP_945146.1, NP_945148.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55065 Da
Antigen Region 26-55 aa
Additional Information
Gene ID 149628
Other Names Pyrin and HIN domain-containing protein 1, Interferon-inducible protein X, PYHIN1, IFIX
Target/Specificity This PYHIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PYHIN1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPYHIN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms IFIX
Function Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.
Cellular Location Isoform 1: Nucleus, nucleoplasm. Isoform 5: Nucleus. Nucleus speckle.
Tissue Location Expressed in spleen, lymph node and peripheral blood leukocytes, and at lower levels in thymus, bone marrow and fetal liver. Down-regulated in breast tumors
Research Areas
Citations (0)

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PYHIN1 belongs to the HIN200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-terminal ends. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis (Ding et al., 2006 [PubMed 16479015]).


Yamaguchi, H., et al. Mol. Carcinog. 47(10):739-743(2008)
Ding, Y., et al. Mol. Cell. Biol. 26(5):1979-1996(2006)
Ding, Y., et al. Oncogene 23(26):4556-4566(2004)

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$ 295.00
$ 99.00
Cat# AP5376a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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