|Application ||WB, IHC-P, FC, E|
|Other Accession||Q16666, NP_945146.1, NP_945148.1|
|Calculated MW||55065 Da|
|Antigen Region||26-55 aa|
|Other Names||Pyrin and HIN domain-containing protein 1, Interferon-inducible protein X, PYHIN1, IFIX|
|Target/Specificity||This PYHIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PYHIN1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PYHIN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.|
|Cellular Location||Isoform 1: Nucleus, nucleoplasm. Isoform 5: Nucleus. Nucleus speckle.|
|Tissue Location||Expressed in spleen, lymph node and peripheral blood leukocytes, and at lower levels in thymus, bone marrow and fetal liver. Down-regulated in breast tumors|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
PYHIN1 belongs to the HIN200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-terminal ends. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis (Ding et al., 2006 [PubMed 16479015]).
Yamaguchi, H., et al. Mol. Carcinog. 47(10):739-743(2008)
Ding, Y., et al. Mol. Cell. Biol. 26(5):1979-1996(2006)
Ding, Y., et al. Oncogene 23(26):4556-4566(2004)
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