|Application ||WB, IHC-P, E|
|Other Accession||B0K036, Q3U276, A8PU71, NP_001036096.1|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||12806 Da|
|Antigen Region||34-62 aa|
|Other Names||Succinate dehydrogenase assembly factor 1, mitochondrial, SDH assembly factor 1, SDHAF1, LYR motif-containing protein 8, SDHF1|
|Target/Specificity||This SDHAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the Central region of human SDHAF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SDHAF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit SDHB of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF3.|
|Cellular Location||Mitochondrion matrix|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).
Ghezzi, D., et al. Nat. Genet. (2009) In press :
Hoffmann, T.W., et al. Transplant. Proc. 41(2):654-656(2009)
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