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CRABP1 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CRABP1 Antibody (C-term) AP5422b
    CRABP1 Antibody (C-term)(Cat. #AP5422b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the CRABP1 antibody detected the CRABP1 protein (arrow).
    detail
  • WB - CRABP1 Antibody (C-term) AP5422b
    Anti-CRABP1 Antibody (C-term) at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - CRABP1 Antibody (C-term) AP5422b
    CRABP1 Antibody (C-term) (Cat. #AP5422b) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRABP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P29762
Other Accession P62966, P62965, P40220, P62964, NP_004369.1
Reactivity Human, Mouse
Predicted Bovine, Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 15566 Da
Antigen Region 90-118 aa
Additional Information
Gene ID 1381
Other Names Cellular retinoic acid-binding protein 1, Cellular retinoic acid-binding protein I, CRABP-I, CRABP1, RBP5
Target/Specificity This CRABP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the C-terminal region of human CRABP1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCRABP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRABP1
Synonyms RBP5
Function Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
Cellular Location Cytoplasm.
Research Areas
Citations (0)
citation

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Background

This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue.

References

Tang, X.H., et al. Exp. Cell Res. 314(1):38-51(2008)
Uhrig, M., et al. BMC Med 6, 38 (2008) :
Guidez, F., et al. Proc. Natl. Acad. Sci. U.S.A. 104(47):18694-18699(2007)
Tanaka, K., et al. Oncogene 26(44):6456-6468(2007)
Wu, Q., et al. Mol. Cancer 6, 45 (2007) :
Flagiello, D., et al. Cytogenet. Cell Genet. 76 (1-2), 17-18 (1997) :
Liu, W., et al. Biochem. Biophys. Res. Commun. 229(3):922-929(1996)
Eller, M.S., et al. Exp. Cell Res. 198(2):328-336(1992)
Astrom, A., et al. J. Biol. Chem. 266(26):17662-17666(1991)
Ong, D.E. Arch Dermatol 123 (12), 1693-1695A (1987) :

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$ 365.00
$ 140.00
Cat# AP5422b
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