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CSAG2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CSAG2 Antibody (N-term) AP5424a
    CSAG2 Antibody (N-term)(Cat. #AP5424a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CSAG2 antibody detected the CSAG2 protein (arrow).
  • IHC-P - CSAG2 Antibody (N-term) AP5424a
    CSAG2 Antibody (N-term) (Cat. #AP5424a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CSAG2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9Y5P2
Other Accession NP_001074317.2, NP_001123300.1, NP_001123298.1, NP_004900.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 1-30 aa
Additional Information
Other Names Chondrosarcoma-associated gene 2/3 protein, Cancer/testis antigen 242, CT242, Taxol-resistant-associated gene 3 protein, TRAG-3, CSAG2, TRAG3
Target/Specificity This CSAG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CSAG2.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCSAG2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSAG2
Synonyms TRAG3
Function Drug-resistance related protein, its expression is associated with the chemotherapy resistant and neoplastic phenotype. May also be linked to the malignant phenotype.
Tissue Location Weakly expressed in kidney. Expressed in various tumor cell lines including carcinomas, myeloid and lymphoid malignancies, melanomas and prostate cancer Overexpressed in taxol-resistant breast cancer line MDA 435TR and the doxorubicin-resistant multiple myelanoma lines RPMI-8226/Dox40 and RPMI-8226/MDR10V EMBL; AF080246; AAC29487.1; -; mRNA EMBL; AF136715; AAD37859.1; -; mRNA EMBL; CH471169; EAW99428.1; -; Genomic_DNA EMBL; BC007228; AAH07228.1; -; mRNA EMBL; BC119734; AAI19735.1; -; mRNA EMBL; BC119735; AAI19736.1; -; mRNA RefSeq; NP_001123298.1; NM_001129826.2. [Q9Y5P2-1] RefSeq; NP_001123300.1; NM_001129828.2. [Q9Y5P2-2] UniGene; Hs.721609; - UniGene; Hs.735281; - UniGene; Hs.741932; - ProteinModelPortal; Q9Y5P2; - iPTMnet; Q9Y5P2; - PhosphoSitePlus; Q9Y5P2; - PRIDE; Q9Y5P2; - DNASU; 389903; - GeneID; 389903; - KEGG; hsa:389903; - CTD; 389903; - DisGeNET; 102723547; - DisGeNET; 389903; - GeneCards; CSAG2; - GeneCards; CSAG3; - H-InvDB; HIX0010307; - H-InvDB; HIX0056157; - H-InvDB; HIX0176527; - H-InvDB; HIX0176530; - HGNC; HGNC:16847; CSAG2 HGNC; HGNC:26237; CSAG3 neXtProt; NX_Q9Y5P2; - PharmGKB; PA134919230; - HOGENOM; HOG000112006; - HOVERGEN; HBG099380; - InParanoid; Q9Y5P2; - PhylomeDB; Q9Y5P2; - GenomeRNAi; 389903; - PRO; PR:Q9Y5P2; - Proteomes; UP000005640; Unplaced CleanEx; HS_CSAG3; - GO; GO:0042493; P:response to drug; TAS:ProtInc 2: Evidence at transcript level; Alternative splicing; Complete proteome; Reference proteome CHAIN 1 127 Chondrosarcoma-associated gene 2/3 protein /FTId=PRO_0000079390 VAR_SEQ 1 67 Missing (in isoform 3) /FTId=VSP_034912 VAR_SEQ 89 105 Missing (in isoform 2) {ECO:0000303|PubMed:15489334, ECO:0000303|Ref.2} /FTId=VSP_004469 VAR_SEQ 94 105 Missing (in isoform 3) /FTId=VSP_034913 CONFLICT 33 33 R -> H (in Ref. 1; AAC29487 and 2; AAD37859). CONFLICT 37 37 L -> H (in Ref. 1; AAC29487 and 2; AAD37859). CONFLICT 58 58 R -> I (in Ref. 1; AAC29487 and 2; AAD37859). CONFLICT 110 110 P -> L (in Ref. 1; AAC29487 and 2; AAD37859). CONFLICT 118 118 K -> E (in Ref. 1; AAC29487 and 2; AAD37859). SEQUENCE 127 AA; 14430 MW; EC1B2C1758129BCC CRC64; MWMGLIQLVE GVKRKDQGFL EKEFYHKTNI KMRCEFLACW PAFTVLGEAW RDQVDWSRLL RDAGLVKMSR KPRASSPLSN NHPPTPKRRG SGRHPLNPGP EALSKFPRQP GREKGPIKEV PGTKGSP
Citations (0)

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References

Hansen, M.A., et al. Mol. Reprod. Dev. 75(2):219-229(2008)
Janjic, B., et al. J. Immunol. 177(4):2717-2727(2006)
Ohta, M., et al. Oncol. Rep. 15(6):1529-1532(2006)
Yao, X., et al. Cancer Genet. Cytogenet. 151(1):1-13(2004)
Lin, C., et al. Gene 285 (1-2), 269-278 (2002) :
Venter, J.C., et al. Science 291(5507):1304-1351(2001)
Feller, A.J., et al. Anticancer Res. 20 (6B), 4147-4151 (2000) :
Duan, Z., et al. Gene 229 (1-2), 75-81 (1999) :

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Cat# AP5424a
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