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IPO7 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - IPO7 Antibody (N-term) AP5434a
    Anti-IPO7 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 120 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - IPO7 Antibody (N-term) AP5434a
    IPO7 Antibody (N-term)(Cat. #AP5434a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the IPO7 antibody detected the IPO7 protein (arrow).
  • WB - IPO7 Antibody (N-term) AP5434a
    IPO7 Antibody (N-term)(Cat. #AP5434a) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the IPO7 antibody detected the IPO7 protein (arrow).
  • IHC-P - IPO7 Antibody (N-term) AP5434a
    IPO7 Antibody (N-term) (Cat. #AP5434a) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CRABP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95373
Other Accession Q9EPL8, NP_006382.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 119517 Da
Antigen Region 142-169 aa
Additional Information
Gene ID 10527
Other Names Importin-7, Imp7, Ran-binding protein 7, RanBP7, IPO7, RANBP7
Target/Specificity This IPO7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-169 amino acids from the N-terminal region of human IPO7.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIPO7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IPO7
Synonyms RANBP7
Function Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
Cellular Location Cytoplasm. Nucleus.
Research Areas
Citations (0)

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The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.


Huang, S., et al. Biosci. Rep. 30(3):159-168(2010)
Chachami, G., et al. Biochem. Biophys. Res. Commun. 390(2):235-240(2009)
Adeyemo, A., et al. PLoS Genet. 5 (7), E1000564 (2009) :
Zaitseva, L., et al. Retrovirology 6, 11 (2009) :
Yao, X., et al. J. Biol. Chem. 283(33):22867-22874(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Dean, K.A., et al. J. Cell. Sci. 114 (PT 19), 3479-3485 (2001) :
Jakel, S., et al. EMBO J. 18(9):2411-2423(1999)
Paraskeva, E., et al. J. Cell Biol. 145(2):255-264(1999)
Jakel, S., et al. EMBO J. 17(15):4491-4502(1998)
Gorlich, D., et al. J. Cell Biol. 138(1):65-80(1997)

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$ 295.00
$ 99.00
Cat# AP5434a
(40 western blots)
Availability: In Stock
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