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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   BUB1A Antibody (N-term)   

BUB1A Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BUB1A Antibody (N-term) AP5438a
    BUB1A Antibody (N-term) (Cat.#AP5438a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the BUB1A antibody detected the BUB1A protein (arrow).
  • FC - BUB1A Antibody (N-term) AP5438a
    BUB1A Antibody (N-term) (Cat. #AP5438a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43683
Other Accession NP_004327
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 122375 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 699
Other Names Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A, BUB1, BUB1L
Target/Specificity This BUB1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BUB1A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBUB1A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BUB1
Synonyms BUB1L
Function Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization (By similarity)
Tissue Location High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index
Research Areas
Citations (0)

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BUB1A is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint.


Gambe, A.E., et al. FEBS Lett. 583(12):1951-1956(2009)
Klebig, C., et al. J. Cell Biol. 185(5):841-858(2009)
Gao, F., et al. Cancer Biol. Ther. 8(7):548-556(2009)
Gao, F., et al. Cancer Biol. Ther. 8(7):627-635(2009)

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$ 295.00
$ 99.00
Cat# AP5438a
(40 western blots)
Availability: In Stock
Bulk Size
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