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DAAM1-T361 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DAAM1-T361 Antibody (N-term) AP5439a
    DAAM1-T361 Antibody (N-term) (Cat.#AP5439a) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).
  • WB - DAAM1-T361 Antibody (N-term) AP5439a
    DAAM1-T361 Antibody (N-term) (Cat.#AP5439a) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the DAAM1 antibody detected the DAAM1 protein (arrow).
  • IHC-P - DAAM1-T361 Antibody (N-term) AP5439a
    DAAM1-T361 Antibody (N-term) (Cat.#AP5439a) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DAAM1-T361 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - DAAM1-T361 Antibody (N-term) AP5439a
    DAAM1-T361 Antibody (N-term) (Cat. #AP5439a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4D1
Other Accession NP_055807.1
Reactivity Human, Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 123473 Da
Antigen Region 341-370 aa
Additional Information
Gene ID 23002
Other Names Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666
Target/Specificity This DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the N-terminal region of human DAAM1-T361.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDAAM1-T361 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAAM1
Synonyms KIAA0666
Function Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.
Cellular Location Cytoplasm. Note=Perinuclear
Tissue Location Expressed in all tissues examined.
Research Areas
Citations (0)

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Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.


Higashi, T., et al. J. Biol. Chem. 283(13):8746-8755(2008)
Liu, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):210-215(2008)
Yamashita, M., et al. Genes Cells 12(11):1255-1265(2007)

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Cat# AP5439a
(40 western blots)
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