|Application ||WB, IHC-P, FC, E|
|Calculated MW||123473 Da|
|Antigen Region||341-370 aa|
|Other Names||Disheveled-associated activator of morphogenesis 1, DAAM1, KIAA0666|
|Target/Specificity||This DAAM1-T361 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the N-terminal region of human DAAM1-T361.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DAAM1-T361 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to disheveled (Dvl) and Rho, and mediates Wnt- induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, cilium basal body. Note=Perinuclear|
|Tissue Location||Expressed in all tissues examined.|
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Provided below are standard protocols that you may find useful for product applications.
Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined.
Higashi, T., et al. J. Biol. Chem. 283(13):8746-8755(2008)
Liu, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):210-215(2008)
Yamashita, M., et al. Genes Cells 12(11):1255-1265(2007)
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