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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PGD Antibody (Center)   

PGD Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PGD Antibody (Center) AP5448c
    PGD Antibody (Center) (Cat.#AP5448c) western blot analysis in 293,MCF-7 cell line lysates (35ug/lane).This demonstrates the PGD antibody detected PGD protein (arrow).
  • WB - PGD Antibody (Center) AP5448c
    PGD Antibody (Center) (Cat.#AP5448c) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PGD antibody detected PGD protein (arrow).
  • IHC-P - PGD Antibody (Center) AP5448c
    PGD Antibody (Center) (Cat. #AP5448c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PGD Antibody (Center) AP5448c
    PGD Antibody (Center) (Cat. #AP5448c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P52209
Other Accession P85968, P14332, Q9DCD0, NP_002622.2, P00349
Reactivity Human, Mouse
Predicted Pig, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 53140 Da
Antigen Region 236-265 aa
Additional Information
Gene ID 5226
Other Names 6-phosphogluconate dehydrogenase, decarboxylating, PGD, PGDH
Target/Specificity This PGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the Central region of human PGD.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGD
Synonyms PGDH
Function Catalyzes the oxidative decarboxylation of 6- phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.

References

Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Kerimov, B.F. Vopr. Med. Khim. 48(5):490-496(2002)
Rippa, M., et al. Biochim. Biophys. Acta 1429(1):83-92(1998)

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$ 295.00
$ 99.00
Cat# AP5448c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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