|Application ||WB, IHC-P, FC, E|
|Calculated MW||35673 Da|
|Antigen Region||3-31 aa|
|Other Names||Retinol dehydrogenase 16, 11--, Microsomal NAD(+)-dependent retinol dehydrogenase 4, RoDH-4, Sterol/retinol dehydrogenase, RDH16, RODH4|
|Target/Specificity||This RDH16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-31 amino acids from the N-terminal region of human RDH16.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RDH16 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oxidoreductase with a preference for NAD. Oxidizes all- trans-retinol and 13-cis-retinol to the corresponding aldehydes. Has higher activity towards CRBP-bound retinol than with free retinol. Oxidizes 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction.|
|Cellular Location||Microsome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein|
|Tissue Location||Highly expressed in adult liver (at protein level). Detected in endometrium, liver and foreskin. Detected in the spineous layers of adult skin, and at lower levels in basal and granular skin layers. Detected in fetal liver and lung|
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Provided below are standard protocols that you may find useful for product applications.
Retinol dehydrogenase 16 (RDH16) is an oxidoreductase with a preference for NAD. It oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. RDH16 has higher activity towards CRBP-bound retinol than with free retinol. It oxidizes 3-alpha-hydroxysteroids and also oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. RDH16 can also catalyze the reverse reaction.
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009) :
Lapshina, E.A., et al. Biochemistry 42(3):776-784(2003)
Cain, J.M., et al. Am. J. Obstet. Gynecol. 186(4):675-683(2002)
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