|Application ||WB, IHC-P, FC, E|
|Calculated MW||74820 Da|
|Antigen Region||204-232 aa|
|Other Names||Spermatogenesis-associated protein 13, APC-stimulated guanine nucleotide exchange factor 2, Asef2, SPATA13|
|Target/Specificity||This SPT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the Central region of human SPT13.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPT13 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up- regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.|
|Cellular Location||Cytoplasm. Cell projection, filopodium. Cell projection, lamellipodium. Cell projection, ruffle membrane Note=Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment|
|Tissue Location||Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle Expression is aberrantly enhanced in most colorectal tumors|
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Provided below are standard protocols that you may find useful for product applications.
The function of SPT13 remains unknown.
Ding, H., et al. Stroke 41(1):177-180(2010)
Bristow, J.M., et al. J. Cell. Sci. 122 (PT 24), 4535-4546 (2009)
Sagara, M., et al. Oncogene 28(10):1357-1365(2009)
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