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MEIS2 Antibody (Center D269)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP5518c 400 µl (40 western blots) In Stock $ 265.00 Add to cart
AP5518c-ev 80 µl (8 western blots) In Stock $ 95.00 Add to cart
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MEIS2 Antibody (Center D269) - Product info

ApplicationWB, IHC, FC
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary AccessionO14770
Other AccessionP97367, NP_733775
ReactivityHuman
PredictedMouse
IsotypeRabbit Ig
Calculated MW51790 Da

MEIS2 Antibody (Center D269) - Additional info

Gene ID 4212
Other Names
MEIS2; MRG1; Homeobox protein Meis2; Meis1-related protein 1
Target/Specificity
This MEIS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-280 amino acids from the Central region of human MEIS2.
Dilution
WB~~1:1000
IHC~~1:50~100
FC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
MEIS2 Antibody (Center D269) is for research use only and not for use in diagnostic or therapeutic procedures.

MEIS2 Antibody (Center D269) - Protein Information

Name MEIS2
Synonyms MRG1
Function
Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3/Meis2B is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoform 3/Meis2B and isoform 4/Meis2D can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription; isoform 5/Meis2E cannot activate DRD1 transcription.
Cellular Location
Nucleus (By similarity).
Tissue Location
Expressed in various tissues. Expressed at high level in the lymphoid organs of hematopoietic tissues. Also expressed in some regions of the brain, such as the putamen

MEIS2 Antibody (Center D269) - Related products

BP5518c: MEIS2 Antibody (Center D269) Blocking peptide

AT2841a: MEIS2 Antibody (monoclonal) (M01)

AT2842a: MEIS2 Antibody (monoclonal) (M03)

MEIS2 Antibody (Center D269) - Application data

  • MEIS2 Antibody (Center D269) (Cat. #AP5518c) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the MEIS2 antibody detected the MEIS2 protein (arrow).

  • MEIS2 Antibody (Center D269) (Cat. #AP5518c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MEIS2 Antibody (Center D269) for immunohistochemistry. Clinical relevance has not been evaluated.

  • MEIS2 Antibody (Center D269) (Cat. #AP5518c) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MEIS2 Antibody (Center D269) - Research Areas

AntibodiesGene OntologyBiological ProcessOrgan DevelopmentPositive Regulation Of Biological ProcessPositive Regulation Of Cellular ProcessPositive Regulation Of Cellular Metabolic ProcessPositive Regulation Of Metabolic ProcessPositive Regulation Of Macromolecule Metabolic ProcessNegative Regulation Of Cellular ProcessNegative Regulation Of Biological ProcessPositive Regulation Of Biosynthetic ProcessPositive Regulation Of Cellular Biosynthetic ProcessSystem DevelopmentMulticellular Organismal DevelopmentPositive Regulation Of Macromolecule Biosynthetic ProcessAnatomical Structure DevelopmentPositive Regulation Of Nitrogen Compound Metabolic ProcessDevelopmental ProcessPositive Regulation Of Nucleobase, Nucleoside, Nucleotide And Nucleic Acid Metabolic ProcessPositive Regulation Of TranscriptionPositive Regulation Of Transcription From RNA Polymerase II PromoterPositive Regulation Of Gene ExpressionPositive Regulation Of Transcription, DNA-dependentPositive Regulation Of RNA Metabolic ProcessNegative Regulation Of Metabolic ProcessNegative Regulation Of Cellular Metabolic ProcessRegulation Of Metabolic ProcessNegative Regulation Of Macromolecule Metabolic ProcessRegulation Of Cellular Metabolic ProcessRegulation Of Transcription From RNA Polymerase II PromoterSensory Organ DevelopmentBiological RegulationRegulation Of Macromolecule Metabolic ProcessNegative Regulation Of Nitrogen Compound Metabolic ProcessRegulation Of Primary Metabolic ProcessRegulation Of Biological ProcessNegative Regulation Of Nucleobase, Nucleoside, Nucleotide And Nucleic Acid Metabolic ProcessEye DevelopmentNegative Regulation Of Cellular Biosynthetic ProcessMulticellular Organismal ProcessNegative Regulation Of Biosynthetic ProcessRegulation Of Cellular ProcessNegative Regulation Of Gene ExpressionNegative Regulation Of TranscriptionNegative Regulation Of Macromolecule Biosynthetic ProcessNegative Regulation Of RNA Metabolic ProcessNegative Regulation Of Transcription, DNA-dependentCellular CompartmentCytoplasmNucleolusNucleusIntracellular PartIntracellularMembrane-bounded OrganelleIntracellular Membrane-bounded OrganelleMembrane-enclosed LumenOrganelle LumenOrganelleIntracellular OrganelleIntracellular Organelle LumenOrganelle PartIntracellular Organelle PartNuclear LumenNuclear PartMolecular FunctionTranscription Cofactor ActivityTranscription Regulator ActivityProtein BindingSequence-specific DNA BindingTranscription Factor BindingTranscription Factor ActivityRNA Polymerase II Transcription Factor ActivityTissueMuscleBrain

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BACKGROUND

MEIS2 is a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs.

REFERENCES

Adkins, D.E., et al. Mol. Psychiatry (2010)
Milech, N., et al. Leuk. Res. 34(3):358-363(2010)
Vasan, R.S., et al. JAMA 302(2):168-178(2009)
Steelman, S., et al. Genome Res. 7(2):142-156(1997)