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AHR Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP5533c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP5533c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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- Backgrounds
AHR Antibody (Center) - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | P35869 |
| Other Accession | NP_001612.1 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 96147 Da |
AHR Antibody (Center) - Additional info | |
| Gene ID 196 | |
| Other Names AHR; BHLHE76; Aryl hydrocarbon receptor; Class E basic helix-loop-helix protein 76 | |
| Target/Specificity This AHR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 562-591 amino acids from the Central region of human AHR. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AHR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AHR Antibody (Center) - Protein Information | |
| Name AHR | |
| Synonyms BHLHE76 | |
| Function Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues | |
| Cellular Location Cytoplasm. Nucleus. Note=Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus | |
| Tissue Location Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle | |
AHR Antibody (Center) - Related products
AP5533c: AHR Antibody (Center)
BP5533c: AHR Antibody (Center) Blocking peptide
AHR Antibody (Center) - Application data
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AHR Antibody (Center) (Cat. #AP5533c) western blot analysis in mouse NIH-3T3(lane 1),T47D cell line(lane 2) lysates (35ug/lane).This demonstrates the AHR antibody detected the AHR protein (arrow).
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AHR Antibody (Center) (Cat. #AP5533c) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AHR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of AHR Antibody (Center)(Cat. #AP5533c) with Hela cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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AHR Antibody (Center) (Cat. #AP5533c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AHR Antibody (Center) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
AHR is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. [provided by RefSeq].
REFERENCES
Davila, S., et al. Genes Immun. (2010) In press : Kalthoff, S., et al. J. Biol. Chem. 285(9):5993-6002(2010) Hall, J.M., et al. Mol. Endocrinol. 24(2):359-369(2010) Schroeder, J.C., et al. Biochemistry 49(2):393-400(2010)
