|Application ||WB, IHC-P, FC, IF, E|
|Calculated MW||69477 Da|
|Antigen Region||407-435 aa|
|Other Names||DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1|
|Target/Specificity||This XRCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human XRCC1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||XRCC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.|
|Cellular Location||Nucleus. Note=Moves from the nucleoli to the global nuclear chromatin upon DNA damage|
|Tissue Location||Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Lamerdin, J.E., et al. Genomics 25(2):547-554(1995)
Gyapay, G., et al. Nat. Genet. 7 (2 SPEC NO), 246-339 (1994) :
Thompson, L.H., et al. Mol. Cell. Biol. 10(12):6160-6171(1990)
Thompson, L.H., et al. Genomics 5(4):670-679(1989)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.