SRD5A2 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P31213 |
Other Accession | NP_000339.2 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 28407 Da |
Antigen Region | 153-182 aa |
Gene ID | 6716 |
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Other Names | 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, SR type 2, Steroid 5-alpha-reductase 2, S5AR 2, Type II 5-alpha reductase, SRD5A2 |
Target/Specificity | This SRD5A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the Central region of human SRD5A2. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SRD5A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SRD5A2 |
---|---|
Function | Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. |
Cellular Location | Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein |
Tissue Location | Expressed in high levels in the prostate and many other androgen-sensitive tissues |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).
References
Thigpen, A.E., et al. N. Engl. J. Med. 327(17):1216-1219(1992)
Thigpen, A.E., et al. J. Clin. Invest. 90(3):799-809(1992)
Labrie, F., et al. Endocrinology 131(3):1571-1573(1992)
Andersson, S., et al. Nature 354(6349):159-161(1991)
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