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IPO9 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IPO9 Antibody (N-term) AP5564a
    IPO9 Antibody (N-term) (Cat. #AP5564a) western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).This demonstrates the IPO9 antibody detected the IPO9 protein (arrow).
  • IHC-P - IPO9 Antibody (N-term) AP5564a
    IPO9 Antibody (N-term) (Cat. #AP5564a) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IPO9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - IPO9 Antibody (N-term) AP5564a
    IPO9 Antibody (N-term) (Cat. #AP5564a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q96P70
Other Accession Q91YE6, NP_060555.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 115963 Da
Antigen Region 79-106 aa
Additional Information
Gene ID 55705
Other Names Importin-9, Imp9, Ran-binding protein 9, RanBP9, IPO9, IMP9, KIAA1192, RANBP9
Target/Specificity This IPO9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-106 amino acids from the N-terminal region of human IPO9.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIPO9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IPO9
Synonyms IMP9, KIAA1192, RANBP9
Function Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
Cellular Location Cytoplasm. Nucleus
Citations (0)

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Background

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.

References

King, F.W., et al. Mol. Cell. Biol. 24(16):7091-7101(2004)
Lubert, E.J., et al. Biochem. Biophys. Res. Commun. 303(3):908-913(2003)
Jakel, S., et al. EMBO J. 21(3):377-386(2002)
Muhlhausser, P., et al. EMBO Rep. 2(8):690-696(2001)

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Cat# AP5564a
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(40 western blots)
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