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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SIA8C Antibody (C-term)   

SIA8C Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SIA8C Antibody  (C-term) AP5596b
    SIA8C Antibody (C-term) (Cat. #AP5596b) western blot analysis in HL-60 cell line lysates (15ug/lane).This demonstrates the SIA8C antibody detected the SIA8C protein (arrow).
  • FC - SIA8C Antibody  (C-term) AP5596b
    SIA8C Antibody (C-term) (Cat. #AP5596b) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O43173
Other Accession Q64689, NP_056963.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43970 Da
Antigen Region 312-340 aa
Additional Information
Gene ID 51046
Other Names Sia-alpha-2, 3-Gal-beta-1, 4-GlcNAc-R:alpha 2, 8-sialyltransferase, 2499-, Alpha-2, 8-sialyltransferase 8C, Alpha-2, 8-sialyltransferase III, ST8 alpha-N-acetyl-neuraminide alpha-2, 8-sialyltransferase 3, Sialyltransferase 8C, SIAT8-C, Sialyltransferase St8Sia III, ST8SiaIII, ST8SIA3, SIAT8C
Target/Specificity This SIA8C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 amino acids from the C-terminal region of human SIA8C.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIA8C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ST8SIA3
Synonyms SIAT8C
Function May transfer sialic acid through alpha-2,8-linkages to alpha-2,3-linked and alpha-2,8-linked sialic acid of N-linked oligosaccharides of glycoproteins and glycolipids. It can form polysialic acid in vitro directly on alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Expressed in fetal and adult brain and fetal liver.
Research Areas
Citations (0)

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Background

ST8SIA3 belongs to a family of sialyltransferases that form sialyl-alpha-2,8-sialyl-R linkages at the nonreducing termini of glycoconjugates (Lee et al., 1998 [PubMed 9826427]).

References

Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Kim, S.J., et al. Biochem. Biophys. Res. Commun. 344(4):1057-1064(2006)
Angata, K., et al. J. Biol. Chem. 275(24):18594-18601(2000)
Lee, Y.C., et al. Arch. Biochem. Biophys. 360(1):41-46(1998)
Zeng, G., et al. Gene 187(1):131-134(1997)

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$ 295.00
$ 99.00
Cat# AP5596b
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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