|Application ||WB, IHC-P, FC, E|
|Antigen Region||62-90 aa|
|Other Names||TATA box-binding protein-associated factor RNA polymerase I subunit D, RNA polymerase I-specific TBP-associated factor 41 kDa, TAFI41, TATA box-binding protein-associated factor 1D, TBP-associated factor 1D, Transcription initiation factor SL1/TIF-IB subunit D, TAF1D, JOSD3|
|Target/Specificity||This TAF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human TAF1D.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TAF1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.|
|Cellular Location||Nucleus EMBL; AK027016; BAB15628.1; -; mRNA EMBL; CR457369; CAG33650.1; -; mRNA EMBL; BC001972; AAH01972.1; -; mRNA CCDS; CCDS8293.1; - RefSeq; NP_077021.1; NM_024116.3 UniGene; Hs.355750; - ProteinModelPortal; Q9H5J8; - SMR; Q9H5J8; - BioGrid; 122548; 54 IntAct; Q9H5J8; 40 MINT; MINT-8247602; - STRING; 9606.ENSP00000314971; - iPTMnet; Q9H5J8; - PhosphoSitePlus; Q9H5J8; - BioMuta; TAF1D; - DMDM; 74733586; - EPD; Q9H5J8; - MaxQB; Q9H5J8; - PaxDb; Q9H5J8; - PeptideAtlas; Q9H5J8; - PRIDE; Q9H5J8; - DNASU; 79101; - Ensembl; ENST00000323981; ENSP00000314971; ENSG00000166012 Ensembl; ENST00000448108; ENSP00000410409; ENSG00000166012 Ensembl; ENST00000526015; ENSP00000435087; ENSG00000166012 GeneID; 79101; - KEGG; hsa:79101; - UCSC; uc001ped.5; human CTD; 79101; - DisGeNET; 79101; - GeneCards; TAF1D; - HGNC; HGNC:28759; TAF1D HPA; HPA055935; - HPA; HPA060385; - MIM; 612823; gene neXtProt; NX_Q9H5J8; - OpenTargets; ENSG00000166012; - PharmGKB; PA164726421; - eggNOG; ENOG410IZI3; Eukaryota eggNOG; ENOG41124VA; LUCA GeneTree; ENSGT00390000009061; - HOGENOM; HOG000154498; - HOVERGEN; HBG096071; - InParanoid; Q9H5J8; - KO; K15215; - OMA; NEDFDSR; - OrthoDB; EOG091G0D0N; - PhylomeDB; Q9H5J8; - TreeFam; TF335756; - BioCyc; ZFISH:ENSG00000166012-MONOMER; - Reactome; R-HSA-427359; SIRT1 negatively regulates rRNA Expression Reactome; R-HSA-427413; NoRC negatively regulates rRNA expression Reactome; R-HSA-5250924; B-WICH complex positively regulates rRNA expression Reactome; R-HSA-73762; RNA Polymerase I Transcription Initiation Reactome; R-HSA-73772; RNA Polymerase I Promoter Escape Reactome; R-HSA-73777; RNA Polymerase I Chain Elongation Reactome; R-HSA-73863; RNA Polymerase I Transcription Termination ChiTaRS; TAF1D; human GenomeRNAi; 79101; - PRO; PR:Q9H5J8; - Proteomes; UP000005640; Chromosome 11 Bgee; ENSG00000166012; - CleanEx; HS_TAF1D; - ExpressionAtlas; Q9H5J8; baseline and differential Genevisible; Q9H5J8; HS GO; GO:0005815; C:microtubule organizing center; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005668; C:RNA polymerase transcription factor SL1 complex; IEA:InterPro GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW GO; GO:0045815; P:positive regulation of gene expression, epigenetic; TAS:Reactome GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW GO; GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome GO; GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome GO; GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome InterPro; IPR027976; TAF1D PANTHER; PTHR14562; PTHR14562; 1 Pfam; PF15333; TAF1D; 1 1: Evidence at protein level; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation CHAIN 1 278 TATA box-binding protein-associated factor RNA polymerase I subunit D /FTId=PRO_0000250717 MOD_RES 23 23 Phosphoserine MOD_RES 138 138 Phosphoserine MOD_RES 234 234 Phosphoserine CONFLICT 14 14 S -> T (in Ref. 3; CAG33650) CONFLICT 97 97 R -> G (in Ref. 3; CAG33650) CONFLICT 278 278 M -> I (in Ref. 3; CAG33650) SEQUENCE 278 AA; 32058 MW; 4BA37C73BB387446 CRC64; MDKSGIDSLD HVTSDAVELA NRSDNSSDSS LFKTQCIPYS PKGEKRNPIR KFVRTPESVH ASDSSSDSSF EPIPLTIKAI FERFKNRKKR YKKKKKRRYQ PTGRPRGRPE GRRNPIYSLI DKKKQFRSRG SGFPFLESEN EKNAPWRKIL TFEQAVARGF FNYIEKLKYE HHLKESLKQM NVGEDLENED FDSRRYKFLD DDGSISPIEE STAEDEDATH LEDNECDIKL AGDSFIVSSE FPVRLSVYLE EEDITEEAAL SKKRATKAKN TGQRGLKM|
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Provided below are standard protocols that you may find useful for product applications.
TAF1D is a member of the SL1 complex, which includes TBP (MIM 600075) and TAF1A (MIM 604903), TAF1B (MIM 604904), and TAF1C (MIM 604905), and plays a role in RNA polymerase I transcription (Wang et al., 2004 [PubMed 15520167]; Gorski et al., 2007 [PubMed 17318177]).
Gorski, J.J., et al. EMBO J. 26(6):1560-1568(2007)
Wang, L., et al. Cancer Res. 64(21):7673-7677(2004)
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