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TAF1D Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TAF1D Antibody  (N-term) AP5597a
    TAF1D Antibody (N-term) (Cat. #AP5597a) western blot analysis in NCI-H292,Jurkat cell line and mouse liver lysates (15ug/lane).This demonstrates the TAF1D antibody detected the TAF1D protein (arrow).
  • IHC-P - TAF1D Antibody  (N-term) AP5597a
    TAF1D Antibody (N-term) (Cat. #AP5597a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF1D Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - TAF1D Antibody  (N-term) AP5597a
    TAF1D Antibody (N-term) (Cat. #AP5597a) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9H5J8
Other Accession NP_077021.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 62-90 aa
Additional Information
Other Names TATA box-binding protein-associated factor RNA polymerase I subunit D, RNA polymerase I-specific TBP-associated factor 41 kDa, TAFI41, TATA box-binding protein-associated factor 1D, TBP-associated factor 1D, Transcription initiation factor SL1/TIF-IB subunit D, TAF1D, JOSD3
Target/Specificity This TAF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human TAF1D.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTAF1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TAF1D
Synonyms JOSD3
Function Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
Cellular Location Nucleus EMBL; AK027016; BAB15628.1; -; mRNA EMBL; CR457369; CAG33650.1; -; mRNA EMBL; BC001972; AAH01972.1; -; mRNA CCDS; CCDS8293.1; - RefSeq; NP_077021.1; NM_024116.3 UniGene; Hs.355750; - ProteinModelPortal; Q9H5J8; - SMR; Q9H5J8; - BioGrid; 122548; 54 IntAct; Q9H5J8; 40 MINT; MINT-8247602; - STRING; 9606.ENSP00000314971; - iPTMnet; Q9H5J8; - PhosphoSitePlus; Q9H5J8; - BioMuta; TAF1D; - DMDM; 74733586; - EPD; Q9H5J8; - MaxQB; Q9H5J8; - PaxDb; Q9H5J8; - PeptideAtlas; Q9H5J8; - PRIDE; Q9H5J8; - DNASU; 79101; - Ensembl; ENST00000323981; ENSP00000314971; ENSG00000166012 Ensembl; ENST00000448108; ENSP00000410409; ENSG00000166012 Ensembl; ENST00000526015; ENSP00000435087; ENSG00000166012 GeneID; 79101; - KEGG; hsa:79101; - UCSC; uc001ped.5; human CTD; 79101; - DisGeNET; 79101; - GeneCards; TAF1D; - HGNC; HGNC:28759; TAF1D HPA; HPA055935; - HPA; HPA060385; - MIM; 612823; gene neXtProt; NX_Q9H5J8; - OpenTargets; ENSG00000166012; - PharmGKB; PA164726421; - eggNOG; ENOG410IZI3; Eukaryota eggNOG; ENOG41124VA; LUCA GeneTree; ENSGT00390000009061; - HOGENOM; HOG000154498; - HOVERGEN; HBG096071; - InParanoid; Q9H5J8; - KO; K15215; - OMA; NEDFDSR; - OrthoDB; EOG091G0D0N; - PhylomeDB; Q9H5J8; - TreeFam; TF335756; - BioCyc; ZFISH:ENSG00000166012-MONOMER; - Reactome; R-HSA-427359; SIRT1 negatively regulates rRNA Expression Reactome; R-HSA-427413; NoRC negatively regulates rRNA expression Reactome; R-HSA-5250924; B-WICH complex positively regulates rRNA expression Reactome; R-HSA-73762; RNA Polymerase I Transcription Initiation Reactome; R-HSA-73772; RNA Polymerase I Promoter Escape Reactome; R-HSA-73777; RNA Polymerase I Chain Elongation Reactome; R-HSA-73863; RNA Polymerase I Transcription Termination ChiTaRS; TAF1D; human GenomeRNAi; 79101; - PRO; PR:Q9H5J8; - Proteomes; UP000005640; Chromosome 11 Bgee; ENSG00000166012; - CleanEx; HS_TAF1D; - ExpressionAtlas; Q9H5J8; baseline and differential Genevisible; Q9H5J8; HS GO; GO:0005815; C:microtubule organizing center; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005668; C:RNA polymerase transcription factor SL1 complex; IEA:InterPro GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW GO; GO:0045815; P:positive regulation of gene expression, epigenetic; TAS:Reactome GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW GO; GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome GO; GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome GO; GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome InterPro; IPR027976; TAF1D PANTHER; PTHR14562; PTHR14562; 1 Pfam; PF15333; TAF1D; 1 1: Evidence at protein level; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation CHAIN 1 278 TATA box-binding protein-associated factor RNA polymerase I subunit D /FTId=PRO_0000250717 MOD_RES 23 23 Phosphoserine MOD_RES 138 138 Phosphoserine MOD_RES 234 234 Phosphoserine CONFLICT 14 14 S -> T (in Ref. 3; CAG33650) CONFLICT 97 97 R -> G (in Ref. 3; CAG33650) CONFLICT 278 278 M -> I (in Ref. 3; CAG33650) SEQUENCE 278 AA; 32058 MW; 4BA37C73BB387446 CRC64; MDKSGIDSLD HVTSDAVELA NRSDNSSDSS LFKTQCIPYS PKGEKRNPIR KFVRTPESVH ASDSSSDSSF EPIPLTIKAI FERFKNRKKR YKKKKKRRYQ PTGRPRGRPE GRRNPIYSLI DKKKQFRSRG SGFPFLESEN EKNAPWRKIL TFEQAVARGF FNYIEKLKYE HHLKESLKQM NVGEDLENED FDSRRYKFLD DDGSISPIEE STAEDEDATH LEDNECDIKL AGDSFIVSSE FPVRLSVYLE EEDITEEAAL SKKRATKAKN TGQRGLKM
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Background

TAF1D is a member of the SL1 complex, which includes TBP (MIM 600075) and TAF1A (MIM 604903), TAF1B (MIM 604904), and TAF1C (MIM 604905), and plays a role in RNA polymerase I transcription (Wang et al., 2004 [PubMed 15520167]; Gorski et al., 2007 [PubMed 17318177]).

References

Gorski, J.J., et al. EMBO J. 26(6):1560-1568(2007)
Wang, L., et al. Cancer Res. 64(21):7673-7677(2004)

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Cat# AP5597a
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