Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Immunology   >   REG3G Antibody (Center)   

REG3G Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - REG3G Antibody  (Center) AP5606c
    AP5606C staining REG3G in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - REG3G Antibody  (Center) AP5606c
    AP5606C staining REG3G in human small intestine sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - REG3G Antibody  (Center) AP5606c
    Anti-REG3G Antibody (Center) at 1:2000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - REG3G Antibody  (Center) AP5606c
    Anti-REG3G Antibody (Center)at 1:1000 dilution + human kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - REG3G Antibody  (Center) AP5606c
    All lanes : Anti-REG3G Antibody (Center) at 1:2000 dilution Lane 1: human kidney lysates Lane 2: human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - REG3G Antibody  (Center) AP5606c
    Anti-REG3G Antibody (Center)at 1:400 dilution + human kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - REG3G Antibody  (Center) AP5606c
    Anti-REG3G Antibody (Center)at 1:1000 dilution + recombinant protein lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - REG3G Antibody  (Center) AP5606c
    All lanes : Anti-REG3G Antibody (Center) at 1:100 dilution Lane 1: human kidney lysates Lane 2: human pancreas lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - REG3G Antibody  (Center) AP5606c
    Western blot analysis of lysate from SH-SY5Y cell line, using REG3G Antibody (Center)(Cat. #AP5606c). AP5606c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - REG3G Antibody  (Center) AP5606c
    REG3G Antibody (Center) (Cat.# AP5606c) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the REG3G antibody detected the REG3G protein (arrow).
  • IHC-P - REG3G Antibody  (Center) AP5606c
    REG3G Antibody (Center) (Cat. #AP5606c) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the REG3G Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - REG3G Antibody  (Center) AP5606c
    Reg3g binds to Staphylococcus aureus and the antibody did block some of this binding(Kindly offered by Dr. Choi).
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q6UW15
Other Accession NP_001008388.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 19330 Da
Antigen Region 89-116 aa
Additional Information
Gene ID 130120
Other Names Regenerating islet-derived protein 3-gamma, REG-3-gamma, Pancreatitis-associated protein 1B, PAP-1B, Pancreatitis-associated protein IB, PAP IB, Regenerating islet-derived protein III-gamma, REG III, Reg III-gamma, Regenerating islet-derived protein 3-gamma 165 kDa form, Regenerating islet-derived protein 3-gamma 15 kDa form, REG3G, PAP1B
Target/Specificity This REG3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-116 amino acids from the Central region of human REG3G.
Dilution IHC-P~~1:50~100
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsREG3G Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name REG3G
Synonyms PAP1B
Function Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
Cellular Location Secreted. Cytoplasm.
Tissue Location Predominantly expressed in pancreas, where it may be restricted to exocrine pancreas. Moderate expression levels in testis and weak in heart, kidney and placenta
Research Areas
Citations ( 0 )

Background

REG3G might be a stress protein involved in the control of bacterial proliferation (By similarity).

References

Taylor-Fishwick, D.A., et al. J. Histochem. Cytochem. 56(2):183-191(2008)
Hamblet, N.S., et al. Pancreas 36(1):1-9(2008)
Hillier, L.W., et al. Nature 434(7034):724-731(2005)
Laurine, E., et al. Biochim. Biophys. Acta 1727(3):177-187(2005)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 295.00
$ 99.00
Cat# AP5606c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions