SLC4A7 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q9Y6M7 |
Other Accession | NP_003606.3 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 136044 Da |
Antigen Region | 1186-1214 aa |
Gene ID | 9497 |
---|---|
Other Names | Sodium bicarbonate cotransporter 3, Electroneutral Na/HCO(3) cotransporter, Sodium bicarbonate cotransporter 2, Sodium bicarbonate cotransporter 2b, Bicarbonate transporter, Solute carrier family 4 member 7, SLC4A7, BT, NBC2, NBC2B, NBC3, NBCn1, SBC2, SLC4A6 |
Target/Specificity | This SLC4A7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1186-1214 amino acids from the C-terminal region of human SLC4A7. |
Dilution | WB~~1:1000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SLC4A7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SLC4A7 |
---|---|
Synonyms | BT, NBC2, NBC2B, NBC3, NBCn1, SBC2, SLC4 |
Function | Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry (PubMed:10347222, PubMed:12403779, PubMed:14736710, PubMed:14578046). Mediates the sodium-dependent bicarbonate transport important for pH recovery after acid load as well as for regulation of steady-state pH in the duodenum and vascular smooth muscle cells (By similarity). Plays a key role in macrophage acidification, mediating bicarbonate import into the cytoplasm which is crucial for net acid extrusion and maintenance of cytoplasmic pH during phagocytosis (PubMed:29779931). Provides cellular bicarbonate for de novo purine and pyrimidine synthesis and is a key mediator of de novo nucleotide synthesis downstream of mTORC1 signaling in proliferating cells (PubMed:35772404). |
Cellular Location | Basolateral cell membrane {ECO:0000250|UniProtKB:Q9R1N3}; Multi-pass membrane protein. Apical cell membrane {ECO:0000250|UniProtKB:Q9R1N3}; Multi-pass membrane protein. Cell projection, stereocilium {ECO:0000250|UniProtKB:Q9R1N3}. Cell membrane; Multi-pass membrane protein. Note=Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells (By similarity). {ECO:0000250|UniProtKB:Q9R1N3} |
Tissue Location | Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. |
Provided below are standard protocols that you may find useful for product applications.
Background
SLC4A7 regulates intracellular pH and may also play a role in bicarbonate salvage in secretory epithelia. Additionally, SLC4A7 may also have associated sodium channel activity.
References
Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010)
Ahmed, S., et al. Nat. Genet. 41(5):585-590(2009)
Ishiguro, H., et al. Addiction 102(8):1320-1325(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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