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ARHH Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP5693c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP5693c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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ARHH Antibody (Center) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q15669 |
| Other Accession | NP_004301.1 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 21331 Da |
ARHH Antibody (Center) - Additional info | |
| Gene ID 399 | |
| Other Names RHOH; ARHH; TTF; Rho-related GTP-binding protein RhoH; GTP-binding protein TTF; Translocation three four protein | |
| Target/Specificity This ARHH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the Central region of human ARHH. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ARHH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ARHH Antibody (Center) - Protein Information | |
| Name RHOH | |
| Synonyms ARHH, TTF | |
| Function Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42 Negatively regulates leukotriene production in neutrophils | |
| Cellular Location Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Note=Co-localizes together with ZAP70 in the immunological synapse (By similarity) | |
| Tissue Location Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis | |
ARHH Antibody (Center) - Related products
AP5693c: ARHH Antibody (Center)
DC03800: Human ARHH cDNA Clone
LY12186a: ARHH Over-expression Lysate
ARHH Antibody (Center) - Application data
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ARHH Antibody (Center) (Cat. #AP5693c) western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the ARHH antibody detected ARHH protein (arrow).
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ARHH Antibody (Center) (Cat. #AP5693c) western blot analysis in mouse spleen tissue lysates (15ug/lane).This demonstrates the ARHH antibody detected ARHH protein (arrow).
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ARHH Antibody (Center) (Cat. #AP5693c) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ARHH Antibody (Center) - Research Areas
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BACKGROUND
RHOH is a member of the Ras superfamily of small GTPases. Expression of a chimeric transcript of LAZ3 and this gene has been reported as a result of the translocation t(3;4) in non-Hodgkin's lymphomas. This gene encodes a small G-like protein, and unlike most other small G proteins which are expressed ubiquitously, this gene is transcribed only in hemopoietic cells.
REFERENCES
Faure, J., et al. Biochimie 83(5):409-414(2001) Yousefi, S., et al. Biochem. Biophys. Res. Commun. 277(2):401-409(2000) Dallery-Prudhomme, E., et al. Genomics 43(1):89-94(1997) Dallery, E., et al. Oncogene 10(11):2171-2178(1995)
