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SPAG1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SPAG1 Antibody (N-term) AP5699a
    SPAG1 Antibody (N-term) (Cat. #AP5699a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SPAG1 antibody detected the SPAG1 protein (arrow).
  • IHC-P - SPAG1 Antibody (N-term) AP5699a
    SPAG1 Antibody (N-term) (Cat. #AP5699a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPAG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q07617
Other Accession NP_003105.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 103639 Da
Antigen Region 124-152 aa
Additional Information
Gene ID 6674
Other Names Sperm-associated antigen 1, HSD-38, Infertility-related sperm protein Spag-1, SPAG1
Target/Specificity This SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of human SPAG1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPAG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPAG1
Function May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity.
Cellular Location Cytoplasm. Note=Colocalizes with tubulin
Tissue Location Present in most tissues, including lung, with the strongest expression in brain, colon, kidney, and testis. In sperm and testis, detected in particular in pachytene primary spermatocytes. Up-regulated in pancreatic tumor tissues and not in normal pancreatic tissue.
Research Areas
Citations (0)

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The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq].


Lin, W., et al. Mol. Hum. Reprod. 7(9):811-818(2001)
Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117(1997)
Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003(1992)

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Cat# AP5699a
(40 western blots)
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