|Application ||WB, IHC-P, E|
|Calculated MW||103639 Da|
|Antigen Region||124-152 aa|
|Other Names||Sperm-associated antigen 1, HSD-38, Infertility-related sperm protein Spag-1, SPAG1|
|Target/Specificity||This SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-152 amino acids from the N-terminal region of human SPAG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPAG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity.|
|Cellular Location||Cytoplasm. Note=Colocalizes with tubulin|
|Tissue Location||Present in most tissues, including lung, with the strongest expression in brain, colon, kidney, and testis. In sperm and testis, detected in particular in pachytene primary spermatocytes. Up-regulated in pancreatic tumor tissues and not in normal pancreatic tissue.|
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Provided below are standard protocols that you may find useful for product applications.
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq].
Lin, W., et al. Mol. Hum. Reprod. 7(9):811-818(2001)
Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117(1997)
Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003(1992)
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