|Application ||WB, IHC-P, FC, E|
|Calculated MW||26171 Da|
|Antigen Region||199-228 aa|
|Other Names||R-spondin-4, Roof plate-specific spondin-4, hRspo4, RSPO4, C20orf182|
|Target/Specificity||This RSPO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human RSPO4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RSPO4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.|
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Provided below are standard protocols that you may find useful for product applications.
RSPO4 is a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
Bergmann, C., et al. Am. J. Hum. Genet. 79(6):1105-1109(2006)
Blaydon, D.C., et al. Nat. Genet. 38(11):1245-1247(2006)
Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)
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