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KIF24 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - KIF24 Antibody (N-term) AP5720A
    KIF24 Antibody (N-term) (Cat. #AP5720a) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the KIF24 antibody detected KIF24 protein (arrow).
  • IHC-P - KIF24 Antibody (N-term) AP5720A
    KIF24 Antibody (N-term) (Cat. #AP5720a) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KIF24 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - KIF24 Antibody (N-term) AP5720A
    KIF24 Antibody (N-term) (Cat. #AP5720a) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q5T7B8
Other Accession Q6NWW5, NP_919289.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 151903 Da
Antigen Region 356-384 aa
Additional Information
Gene ID 347240
Other Names Kinesin-like protein KIF24, KIF24, C9orf48
Target/Specificity This KIF24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 356-384 amino acids from the N-terminal region of human KIF24.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKIF24 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIF24
Synonyms C9orf48
Function Microtubule-dependent motor protein that acts as a negative regulator of ciliogenesis by mediating recruitment of CCP110 to mother centriole in cycling cells, leading to restrict nucleation of cilia at centrioles. Mediates depolymerization of microtubules of centriolar origin, possibly to suppress aberrant cilia formation (PubMed:21620453). Following activation by NEK2 involved in disassembly of primary cilium during G2/M phase but does not disassemble fully formed ciliary axonemes. As cilium assembly and disassembly is proposed to coexist in a dynamic equilibrium may suppress nascent cilium assembly and, potentially, ciliar re-assembly in cells that have already disassembled their cilia ensuring the completion of cilium removal in the later stages of the cell cycle (PubMed:26290419).
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Primarily localizes to the mother centriole/basal body and is either absent at daughter centriole
Citations (0)

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References

Tsuritani, K., et al. Genome Res. 17(7):1005-1014(2007)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
Miki, H., et al. Proc. Natl. Acad. Sci. U.S.A. 98(13):7004-7011(2001)

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Cat# AP5720A
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(40 western blots)
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