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TUBB3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TUBB3 Antibody (N-term) AP5752A
    All lanes : Anti-TUBB3 Antibody (N-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TUBB3 Antibody (N-term) AP5752A
    TUBB3 Antibody (N-term) (Cat. #AP5752a) western blot analysis in mouse brain tissue lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
  • WB - TUBB3 Antibody (N-term) AP5752A
    TUBB3 Antibody (N-term) (Cat. #AP5752a) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the TUBB3 antibody detected the TUBB3 protein (arrow).
  • IHC-P - TUBB3 Antibody (N-term) AP5752A
    TUBB3 Antibody (N-term) (Cat. #AP5752a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TUBB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - TUBB3 Antibody (N-term) AP5752A
    TUBB3 Antibody (N-term) (Cat. #AP5752a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13509
Other Accession P09652, Q4QRB4, Q9ERD7, Q60HC2, Q2T9S0, NP_006077.2
Reactivity Human, Mouse
Predicted Rat, Monkey, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50433 Da
Antigen Region 36-63 aa
Additional Information
Gene ID 10381
Other Names Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3, TUBB4
Target/Specificity This TUBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human TUBB3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB3
Synonyms TUBB4
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Research Areas
Citations ( 0 )


Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.


Khan, I.A., et al. Biochemistry 35(12):3704-3711(1996)
Khan, I.A., et al. Biochemistry 35(12):3704-3711(1996)
Vinores, S.A., et al. Exp. Eye Res. 60(4):385-400(1995)

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$ 295.00
$ 99.00
Cat# AP5752A
(40 western blots)
Availability: In Stock
Bulk Size
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