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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VLDLR Antibody (Center)   

VLDLR Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - VLDLR Antibody (Center) AP5754c
    Overlay histogram showing THP-1 cells stained with AP5754c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5754c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - VLDLR Antibody (Center) AP5754c
    AP5754c staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - VLDLR Antibody (Center) AP5754c
    AP5754c staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - VLDLR Antibody (Center) AP5754c
    Anti-VLDLR Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 96 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - VLDLR Antibody (Center) AP5754c
    Anti-VLDLR Antibody (Center) at 1:2000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 96 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - VLDLR Antibody (Center) AP5754c
    VLDLR Antibody (Center) (Cat. #AP5754c) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VLDLR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P98155
Other Accession P35953, NP_003374.3
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 96098 Da
Antigen Region 484-510 aa
Additional Information
Gene ID 7436
Other Names Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR
Target/Specificity This VLDLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-510 amino acids from the Central region of human VLDLR.
Dilution FC~~1:25
IHC-P~~1:10~50
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVLDLR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VLDLR
Function Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein
Tissue Location Abundant in heart and skeletal muscle; also ovary and kidney; not in liver
Research Areas
Citations ( 0 )

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq].

References

Sakai, K., et al. Brain Res. 1276, 11-21 (2009)
Francis, P.J., et al. J. Med. Genet. 46(5):300-307(2009)
Ananyeva, N.M., et al. Blood Coagul. Fibrinolysis 19(6):543-555(2008)
Turkmen, S., et al. Eur. J. Hum. Genet. 16(9):1070-1074(2008)

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$ 295.00
$ 99.00
Cat# AP5754c
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(40 western blots)
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