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TCF4 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | P15884 |
| Other Accession | Q60722, NP_003190.1 |
| Reactivity | Human, Mouse |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 71308 Da |
| Antigen Region | 48-73 aa |
| Gene ID | 6925 |
|---|---|
| Other Names | Transcription factor 4, TCF-4, Class B basic helix-loop-helix protein 19, bHLHb19, Immunoglobulin transcription factor 2, ITF-2, SL3-3 enhancer factor 2, SEF-2, TCF4, BHLHB19, ITF2, SEF2 |
| Target/Specificity | This TCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-73 amino acids from the N-terminal region of human TCF4. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TCF4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TCF4 |
|---|---|
| Synonyms | BHLHB19, ITF2, SEF2 |
| Function | Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'- CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'- CCANNTGG-3'. |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:22777675} |
| Tissue Location | Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
TCF4 is transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Defects in this gene are a cause of Pitt-Hopkins syndrome.
References
Rosenfeld, J.A., et al. Genet. Med. 11(11):797-805(2009)
Herbst, A., et al. Biochem. Biophys. Res. Commun. 387(4):736-740(2009)
Rena, V., et al. Placenta 30(10):876-883(2009)
Henthorn, P., et al. Science 247(4941):467-470(1990)
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