- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Other Accession||Q91X46, Q9N0A8, Q5ZLX4, NP_062455.1|
|Calculated MW||59783 Da|
|Antigen Region||211-240 aa|
|Other Names||Rho guanine nucleotide exchange factor 3, Exchange factor found in platelets and leukemic and neuronal tissues, XPLN, ARHGEF3|
|Target/Specificity||This ARHGEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human ARHGEF3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ARHGEF3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.|
|Tissue Location||Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney.|
Provided below are standard protocols that you may find useful for product applications.
Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Arthur, W.T., et al. J. Biol. Chem. 277(45):42964-42972(2002)
Harrington, A.W., et al. J. Neurosci. 22(1):156-166(2002)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Thiesen, S., et al. Biochem. Biophys. Res. Commun. 273(1):364-369(2000)
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