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ATP1A2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ATP1A2 Antibody (Center) AP5828c
    Anti-ATP1A2 Antibody (Center) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 112 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - ATP1A2 Antibody (Center) AP5828c
    Confocal immunofluorescent analysis of ATP1A2 Antibody (Center)(Cat. #AP5828c) with MCF-7 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • WB - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the ATP1A2 antibody detected ATP1A2 protein (arrow).
  • WB - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) western blot analysis in MCF-7 cell line lysates (15ug/lane).This demonstrates the ATP1A2 antibody detected ATP1A2 protein (arrow).
  • IHC-P - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP1A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P50993
Other Accession P06686, D2WKD8, Q6PIE5, A2VDL6, NP_000693.1
Reactivity Human, Mouse
Predicted Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 112265 Da
Antigen Region 451-479 aa
Additional Information
Gene ID 477
Other Names Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Sodium pump subunit alpha-2, ATP1A2, KIAA0778
Target/Specificity This ATP1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human ATP1A2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP1A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP1A2
Synonyms KIAA0778
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients.
Cellular Location Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Research Areas
Citations ( 0 )
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Cat# AP5828c
(40 western blots)

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