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SAMSN1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SAMSN1 Antibody (Center) AP5877c
    SAMSN1 Antibody (Center) (Cat. #AP5877c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SAMSN1 antibody detected the SAMSN1 protein (arrow).
    detail
  • IHC-P - SAMSN1 Antibody (Center) AP5877c
    SAMSN1 Antibody (Center) (Cat. #AP5877c) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SAMSN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - SAMSN1 Antibody (Center) AP5877c
    SAMSN1 Antibody (Center) (Cat. #AP5877c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, FC, WB, E
Primary Accession Q9NSI8
Other Accession NP_071419.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 41708 Da
Antigen Region 255-283 aa
Additional Information
Gene ID 64092
Other Names SAM domain-containing protein SAMSN-1, Hematopoietic adaptor containing SH3 and SAM domains 1, Nash1, SAM domain, SH3 domain and nuclear localization signals protein 1, SH3-SAM adaptor protein, SAMSN1, HACS1
Target/Specificity This SAMSN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-283 amino acids from the Central region of human SAMSN1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSAMSN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SAMSN1
Synonyms HACS1
Function Negative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation (By similarity).
Cellular Location Nucleus. Cytoplasm. Cell projection, ruffle. Note=Shuttles between cytoplasm and nucleus. Colocalizes with the actin cytoskeleton and actin-rich membrane ruffles (By similarity).
Tissue Location Detected in peripheral blood B-cells (at protein level). Detected in spleen, liver and peripheral blood
Research Areas
Citations (0)
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$ 182.50
$ 70.00
Cat# AP5877c
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