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SIAH2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SIAH2 Antibody (Center) AP5878c
    SIAH2 Antibody (Center) (Cat. #AP5878c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the SIAH2 antibody detected the SIAH2 protein (arrow).
  • IHC-P - SIAH2 Antibody (Center) AP5878c
    SIAH2 Antibody (Center) (Cat. #AP5878c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SIAH2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - SIAH2 Antibody (Center) AP5878c
    SIAH2 Antibody (Center) (Cat. #AP5878c) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43255
Other Accession Q9I8X5, Q8R4T2, Q06986, Q7SYL3, NP_005058.3
Reactivity Human
Predicted Zebrafish, Mouse, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34615 Da
Antigen Region 216-244 aa
Additional Information
Gene ID 6478
Other Names E3 ubiquitin-protein ligase SIAH2, 632-, Seven in absentia homolog 2, Siah-2, hSiah2, SIAH2
Target/Specificity This SIAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-244 amino acids from the Central region of human SIAH2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIAH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIAH2
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear
Tissue Location Widely expressed at low level.
Research Areas
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Cat# AP5878c
(40 western blots)
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