|Application ||WB, IHC-P, FC, E|
|Other Accession||Q9I8X5, Q8R4T2, Q06986, Q7SYL3, NP_005058.3|
|Predicted||Zebrafish, Mouse, Rat, Xenopus|
|Calculated MW||34615 Da|
|Antigen Region||216-244 aa|
|Other Names||E3 ubiquitin-protein ligase SIAH2, 632-, Seven in absentia homolog 2, Siah-2, hSiah2, SIAH2|
|Target/Specificity||This SIAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-244 amino acids from the Central region of human SIAH2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Precautions||SIAH2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not. Promotes monoubiquitination of SNCA.|
|Cellular Location||Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear|
|Tissue Location||Widely expressed at low level.|
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