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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   HLA-F Antibody (Center)   

HLA-F Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HLA-F Antibody (Center) AP5881c
    All lanes : Anti-HLA-F Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HLA-F Antibody (Center) AP5881c
    HLA-F Antibody (Center) (Cat. #AP5881c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HLA-F antibody detected the HLA-F protein (arrow).
  • FC - HLA-F Antibody (Center) AP5881c
    HLA-F Antibody (Center) (Cat. #AP5881c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30511
Other Accession NP_001091948.1, NP_061823.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39062 Da
Antigen Region 172-198 aa
Additional Information
Gene ID 3134
Other Names HLA class I histocompatibility antigen, alpha chain F, CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F, HLA-F, HLA-54, HLAF
Target/Specificity This HLA-F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-198 amino acids from the Central region of human HLA-F.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-F Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HLA-F
Synonyms HLA-5.4, HLAF
Function Involved in the presentation of foreign antigens to the immune system.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
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$ 295.00
$ 99.00
Cat# AP5881c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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