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NCF1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NCF1 Antibody (N-term) AP5882a
    NCF1 Antibody (N-term) (Cat. #AP5882a) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the NCF1 antibody detected the NCF1 protein (arrow).
    detail
  • IHC-P - NCF1 Antibody (N-term) AP5882a
    NCF1 Antibody (N-term) (Cat. #AP5882a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NCF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P14598
Other Accession O77774, A8MVU1, A6NI72, NP_000256.3
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 44682 Da
Antigen Region 26-52 aa
Additional Information
Gene ID 653361
Other Names Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A
Target/Specificity This NCF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-52 amino acids from the N-terminal region of human NCF1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCF1
Synonyms NOXO2, SH3PXD1A
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Detected in peripheral blood monocytes and neutrophils (at protein level).
Research Areas
Citations (0)
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$ 182.50
$ 70.00
Cat# AP5882a
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