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ILKAP Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ILKAP Antibody (Center) AP5884c
    ILKAP Antibody (Center) (Cat. #AP5884c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ILKAP antibody detected the ILKAP protein (arrow).
    detail
  • WB - ILKAP Antibody (Center) AP5884c
    ILKAP Antibody (Center) (Cat. #AP5884c) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ILKAP antibody detected the ILKAP protein (arrow).
    detail
  • WB - ILKAP Antibody (Center) AP5884c
    Western blot analysis of ILKAP (arrow) using rabbit polyclonal ILKAP Antibody (Center) (Cat. #AP5884c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILKAP gene.
    detail
  • IHC-P - ILKAP Antibody (Center) AP5884c
    ILKAP Antibody (Center) (Cat. #AP5884c) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ILKAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9H0C8
Other Accession Q9Z1Z6, Q8R0F6, Q0IIF0, NP_110395.1
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 42907 Da
Antigen Region 243-270 aa
Additional Information
Gene ID 80895
Other Names Integrin-linked kinase-associated serine/threonine phosphatase 2C, ILKAP, ILKAP
Target/Specificity This ILKAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the Central region of human ILKAP.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsILKAP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ILKAP
Function Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.
Cellular Location Cytoplasm.
Tissue Location Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues.
Research Areas
Citations (0)
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$ 182.50
$ 70.00
Cat# AP5884c
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