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PENK Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PENK Antibody (Center) AP5889C
    PENK Antibody (Center) (Cat. #AP5889c) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the PENK antibody detected the PENK protein (arrow).
  • IHC-P - PENK Antibody (Center) AP5889C
    PENK antibody (Center) (Cat. #AP5889c) immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PENK antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PENK Antibody (Center) AP5889C
    PENK Antibody (Center) (Cat. #AP5889c) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01210
Other Accession NP_001129162.1, NP_006202.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30787 Da
Antigen Region 145-174 aa
Additional Information
Gene ID 5179
Other Names Proenkephalin-A, Synenkephalin, Met-enkephalin, Opioid growth factor, OGF, PENK(114-133), PENK(143-183), Met-enkephalin-Arg-Gly-Leu, Leu-enkephalin, PENK(237-258), Met-enkephalin-Arg-Phe, PENK
Target/Specificity This PENK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human PENK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPENK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
Cellular Location Secreted.
Research Areas
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Cat# AP5889C
(40 western blots)
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